Fig. 6. The anti-proliferative activity of MI-743 on gastric cancer xenografts.

Gastric cancer cells MGC-803 were transplanted subcutaneously to the right side of BALB-C nude mice. When most of the tumor volumes reached 100 mm³, the mice were treated with oil or compound MI-743(40 mg/kg, once a day) for 25 days. a The average tumor weight. b The tumor volumes were measured every 4 days. (c) The mice body weights were measured every 2 days. d, e The tumor tissues were collected and fixed in 4% buffered paraformaldehyde and paraffin-embedded for immunohistochemistry to detect the protein level of 8-oxo-dG and ATMpS1981 (e), H&E staining to observe the cell morphology and TUNEL staining to examine apoptosis status (d). Pictures for immunohistochemistry and H&E staining were originally captured at ×200 and ×400 magnification. Pictures for TUNEL staining were originally captured at ×400 magnification. f The mice major organs were collected and fixed in 4% buffered paraformaldehyde and paraffin-embedded for H&E staining. Pictures were originally captured at 200 × magnification using microscope. *P < 0.05, **P < 0.01 or ***P < 0.001 compared with the controls