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. 2019 Jun 3;9:8245. doi: 10.1038/s41598-019-44670-w

Table 1.

Sequences and predicted specificity of primers used for qPCR validation of array findings.

Gene symbol Primer sequence (5′-3′) Ovis Accession Predicted specificity
STARD10 F-aagagctgcgtcatcacctac XM_012155230 MAP exposed
R-gtgtagccacggtttgaagt
BLA-DQB F-cagatcaaggttcggtggtt XM_012173129 MAP exposed
R-ctctgggcagattcagact
ITGB3 F-agcagggtgaacccctaaag XM_012186283 Diseased
R-ggcatttaggtcaaccagga
LXN F-taatagccgtctgccaaagg XM_004003214.3 Diseased
R-ggcaccactgctgttgataa
FCN1 F-cacagttagaggagctctcg XM_012117952 Diseased
R-ccgatttgccaagtaccagt
CES1 F-gacaaggaagtggctttctggaat XM_004014994 Diseased
R-acgcctctttggcacataaa
MYH9 (FST) F-ggattctctgctgcctgttc XM_015094855.1 Multibacillary
R-cctcaccgatgtcttcatca
DLGAP4 F-actggttcctgaagctgctc XM_015099858 Multibacillary
R-ctggaatttctgcgacatca
BPI F-aagctgctcctggaactgaa NM_001306124 Paucibacillary
R-ggacatctgcaccgaacag
C10H15orf48 F-gcttcgtcattcgctgtgta XM_004010648 Resilient
R-cggaccttctgcaactcttc
DHRS7 F-atggccaatgatttgaagga XM_004010693 Resilient
R-cagtggcttgaaaaatgcaa
GNG11 F-tcgtttgttgtcgttttcca NM_001139445 Resilient
R-gtcatgcacaagtggtgtcc
TRMT10B F-gaaggcccaagaacactctg XM_004004242.3 Resilient
R-gctgcctccttaggctctct
Ovine B-Actin F-catcctgaccctcaagtacc NM_001009784.2 Reference gene
R-tcgttgtagaaggtgtggtg
Ovine GAPDH F-agaaacctgccaagtatgatg NM_001190390.1 Reference gene
R-cctagaatgcccttgagagg