Fig. 3.

miR-328 negatively targets FAM13A. a The potential binding sites for the interaction between miR-328 and FAM13A. b The luciferase activity of FAM13A-WT and FAM13A-MUT with or without the cotransfection of miR-328. * p < 0.05 vs. the NC group. c The mRNA level of FAM13A after altering miR-328 activity. d, e The protein level of FAM13A after altering miR-328 expression. *p < 0.05 vs. the mimic NC group; ^#p < 0.05 vs. the inhibitor NC group. miR-328, microRNA-328; FAM13A, family with sequence similarity 13, member A; RT-qPCR, reverse transcription quantitative polymerase chain reaction; NC, negative control; WT, wild type; and MUT, mutant type. The results were measurement data and are expressed as the mean ± standard deviation. The data in Fig. 3b were analyzed using an unpaired t-test, and the data in Fig. 3c and e were analyzed using one-way analysis of variance; the experiment was conducted in triplicate