Table 1.
Primer sequences for RT-qPCR
| Gene | Primer sequence (5’-3’) |
|---|---|
| miR-328 | F: ACCCCGTCCCCCCGTC |
| R: ACAGACAGCATCACTCA | |
| FAM13A | F: GATGGTGGACACACTCAGCA |
| R: CGCCGCCTCATGAAAGAATG | |
| Collagen 1 A | F: GCAATGCTGAATCGTCCCAC |
| R: CAGCACAGGCCCTCAAAAAC | |
| Collagen 3 A | F: TCACCACCACTGTCACGATG |
| R: GTCACACAACGGAAGTTGGC | |
| α-SMA | F: TGGCCACTGCTGCTTCCTCT |
| R: GGGGCCAGCTTCGTCATACTCCT | |
| U6 | F: GCTTCGGCAGCACATATACTAAAAT |
| R: CGCTTCACGAATTTGCGTGTCAT | |
| GAPDH | F: TCCCTCAAGATTGTCAGCAATG |
| R: AGATCCACAACGGATACATTGG |
RT-qPCR reverse transcription quantitative polymerase chain reaction, miR-328, microRNA-328; FAM13A, family with sequence similarity 13, member A; α-SMA, α-smooth muscle actin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; F, forward; and R, reverse