Fig. 5.

CaMKK2 links tumor factor signaling to AMPK activation. WT and Camkk2^−/− BMDM were generated in regular differentiation medium in the presence or absence of E0771-conditioned medium (RM and TCM, respectively). a CaMKK2 and phospho-CaMK1 immunoblots. b Expression of phosphorylated (T-172; p-AMPK) and the total AMPKα was assessed by immunoblot. c Quantification of p-AMPK/AMPK ratio (N = 3 biological replicates; mean ± SEM). Each lane refers to protein lysate extracted from a single biological replicate. A t test was used to calculate p-values. Asterisks refer to *p < 0.05, **p < 0.01, and ***p < 0.005, respectively