Fig. 6.

Phenotype of WT and Camkk2^−/− BMDM generated in the presence or absence of tumor-conditioned medium. WT and Camkk2^−/− BMDM were generated in the presence of RM or TCM. a Left: CD206 and MHC II expression on CD11b⁺ F4/80⁺ gated BMDM. Right: percentages of CD206⁺/MHC II⁻ and CD206⁻/MHC II⁺ subsets (mean ± SEM; N = 3 biological replicates). b Expression of MHC II on CD11b + F4/80 + gated BMDM (mean of MFI ± SEM; N = 3 biological replicates). c Cxcl9 expression (mean ± SEM; N = 3 biological replicates). These experiments were replicated at least three times. d Tumors of comparable size (500–700 mm³) were removed from WT and Camkk2^−/− mice, and myeloid cells were then identified and sorted by flow cytometry. Bar graph reports mean ± SEM of CD206⁺/MHC II⁻ and CD206⁻/MHC II⁺ TAM percentages gated on “Mac” subset in the Supplementary Fig. 2 (N = 5 and 4 tumors removed from WT and Camkk2^−/− mice, respectively). e Cxcl9 expression in TAM sorted from E0771 tumors removed from WT and Camkk2^−/− mice (mean ± SEM; N = 3 and 3 tumors for each genotype)