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. 2019 Jun 3;9:8160. doi: 10.1038/s41598-019-44480-0

Figure 2.

Figure 2

The characterization of B2T-mBax mosquitoes. (a) The gene construct derived from the piggyBac-based vector, pBac[pB2T-mBax; 3xP3-EGFP]. The T7-mBax gene is expressed under the control of An. stephensi b2t gene promoter and An. gambiae trypsin terminator. (b) Detection of the T7-mBax protein in the testes of B2T-mBax mosquitoes by immunoblotting with anti-T7 antibodies. An anti-alpha tubulin antibody was used as the loading control. Testes and accessory glands of mosquitoes 12-hour post eclosion were used for analysis. (c) Gonads of 1-day old adult male wild-type mosquitoes and (c’) B2T-mBax mosquitoes. (d) Gonads of 1-day old adult male wild-type mosquitoes and (d’) B2T-mBax mosquitoes stained with trypan blue. (e,e’) The magnified images of the indicated testes stained with trypan blue. Scale bars = 100 μm. (f) Comparison of survival rates between B2T-mBax and wild-type mosquito males. Mosquitoes immediately after eclosion were used in analyses. Transgenic and wild type mosquitoes were taken from a single heterogeneous strain using EGFP selection, respectively. The survival curves of the groups were estimated by Kaplan-Meier methods. No significant differences were observed between B2T-mBax males and wild-type males. (line D2; P = 0.9725, line D3; P = 0.4049, and line F2; P = 0.6079, calculated by the Log-rank test).