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. 2019 Jun 3;9:8215. doi: 10.1038/s41598-019-44696-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Platelet endothelial cell adhesion molecule (PECAM-1) staining in the retina. (A) Representative images of PECAM-1 ocular sections of flight (FLT) and ground control (GC) mice. PECAM-1 positive cells were identified with red fluorescence, endothelium was stained with lectin (green). The nuclei of photoreceptors were counterstained with DAPI (blue). In the control retinal tissue, only some positive cells were found. In the retina from flight mice, enhanced immunoreactivity of PECAM cells was apparent in the retinal inner nuclear layer (INL) and ganglion cell layers (GCL). Scale bar = 50 μm. (B) Immunoreactivity of PECAM-1 staining in the retina. The average fluorescence intensity for PECAM-1 activity was measured and calculated using the ImageJ program. Fluorescence was averaged across 5 retinas per group. Values are represented as mean density ± SEM. ^*Significantly higher than GC group (p < 0.05).