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. 2019 Jun 4;10(6):435. doi: 10.1038/s41419-019-1659-1

Fig. 2. STYK1 overexpression facilitated NSCLC cells proliferation.

Fig. 2

a The growth curves of H1299 and Calu-1 cells. Cell viabilities were detected by CCK-8 assay and expressed as OD values. b Representative images and results of EdU incorporation assay. The result was calculated as the ratio between the number of EdU-stained cells (red fluorescence) and the total number of Hoechst 33342-stained cells (blue fluorescence). c The representative images and results of colony formation assay. The stained colonies were solubilized with 30% acetic acid; the absorbances were read at 540 nm and normalized to the NC group. d Photographs showing tumor xenograft morphologies in each group, changes in body weight of nude mice, and tumor growth curve drawn from the tumor volumes after subcutaneously injection of NC or STYK1 OE H1299 cells, respectively. *P < 0.05 vs. the NC group