Fig. 5. SPINT2 was essential for STYK1-mediated NSCLC cell proliferation, migration, and invasion.

a The growth curves of H1299 and Calu-1 cells. Cell viabilities were detected by CCK-8 assay and expressed as OD values. b The representative results of colony formation assay. Stained colonies were solubilized with 30% acetic acid; the absorbances were read at 540 nm and normalized to the Vector1 group. c Representative western blot results of STYK1, SPINT2, E-cadherin, Snail were shown. Membranes were re-probed for β-actin expression to show that similar amounts of protein were loaded in each lane. d Representative images and results of transwell migration and invasion assay. e Photographs showing tumor xenograft morphologies in each group, f changes in body weight of nude mice, and tumor growth curve drawn from the tumor volumes after subcutaneously injection of STYK1 OE + Vector2 and STYK1 OE + SPINT2 OE H1299 cells, respectively. g Representative western blot results of STYK1, SPITN2, E-cadherin, Snail were shown. Membranes were re-probed for Tubulin expression to show that similar amounts of protein were loaded in each lane. ^*P < 0.05 vs. the Vector1 group, ^#P < 0.05 vs. the STYK1 OE + Vector2 group