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. 2019 Apr 9;47(10):5074–5085. doi: 10.1093/nar/gkz256

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Verification of DNA–SOD1 interactions in vitro. (A) The selected DNA sequences (S1–S7, 12 bp) with different SOD1 binding ability. (B and C) EMSA showed that the formation of S1-SOD1 complexes was dependent on concentrations of SOD1 in 10 mM pH 7.4 PBS, and pH did not impact the binding of SOD1 to DNA in the tested range of pH. (D) EMSA indicated that SOD1 binds to the selected DNA sequences with different preference under tested conditions. (E and F) The competition performed with excess of the unlabelled S1 and S2 further confirms the formation of S1-SOD1 complexes.

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