Figure 7.

Heat map plot of directed global significance score and multiplex quantification of cytokines and chemokines using the multiplex map rat cytokine/chemokine magnetic bead 27‐plex immunoassays. (A) The map displays the extent to which a gene set's genes are up‐ or downregulated with the variables showing the significantly expressed immune pathways in LCTCs, BCTCs, and LMNs compared to primary tumors. (B) A panel of cytokines, chemokines, and growth factor were measured in lymph from metastatic tumor‐bearing animals relative to that of the lymph from normal animals. (C) Absolute mean concentration pg·mL⁻¹ of cytokines, chemokines, and growth factors in the lymph of metastatic tumor‐bearing and nonmetastatic tumor‐bearing animals. (D) Absolute mean concentration pg·mL⁻¹ of cytokines and chemokines released by LCTCs, BCTCs, and MTLn3 cells detected in 3D culture medium supernatants. (E) CXCR4, CX3CR1, and CXCR3 expression in LCTCs and BCTCs was detected by immunocytochemistry, and the results were visualized by confocal microscopy. (F) Immunoblotting of EGFR, CXCR3, CX3CR1, and CXCR4. β‐actin used as loading control. n=three samples from three animals.