Figure 2.

STAT3 is differentially expressed and activated by OSM and LIF. Mouse calvarial osteoblasts were cultured in the absence (Co) or presence of LIF or OSM (both at 100 ng/mL) for 48 h to assess Stat3 mRNA expression by RT-qPCR (A) or for 15 and 30 min to assess phosphorylated and total STAT3 protein levels by Western Blot (B). Values in (A) represent means for four wells and SEM is shown as vertical bars. Significant differences compared to untreated cells (Co) is defined as ^***P < 0.001 analyzed by one-way ANOVA followed by Dunnet's multiple comparison test vs. Co.