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. 2019 May 28;10:1164. doi: 10.3389/fimmu.2019.01164

Figure 7.

Figure 7

Lack of effect by OSM and LIF on osteoclast formation in bone marrow macrophage cultures. (A,B) BMMs were cultured in M-CSF (30 ng/mL) or differentiated to osteoclasts by addition of M-CSF + RANKL (30 and 4 ng/mL, respectively) in the presence or absence of LIF or OSM (both at 100 ng/mL) for 3 days before staining for tartarate-resistant acid phosphatase (TRAP)-positive multinucleated osteoclasts (TRAP+MuOCL), which then were counted. No osteoclasts were formed in the absence of RANKL. (C) Quantitative PCR analyses of Il6st, Lifr, and Acp5 in BMM cultured for 72 h with M-CSF (30 ng/ml) or M-CSF+RANKL (30 and 4 ng/ml, respectively). Values represent means for four wells and SEM is shown as vertical bars. In (C) ***, indicates significant difference compared to cells treated with M-CSF (M), P < 0.001. In (B) Statistical significance was determined one-way ANOVA followed by Tukey's post-hoc test and in (C), Student's t-test was used for comparison between M and M + R groups for each gene.