Figure 1.

Predominance of CCR6⁺CXCR3-CD4⁺ T cells in mLNs of CD when compared to UC patients. (A) CD3⁺ T cells isolated from mLNs of CD and UC patients were concatenated for t-SNE analysis. Feature plots of the indicated antigens (left panels). Frequencies of CD4⁺ and memory CD45RA⁻ T cells (right panels). (B) Representative dot plots of CCR6⁺CXCR3⁻, CCR6⁺CXCR3⁺ and CCR6⁻CXCR3⁺ CD4⁺ subsets, (C) their expression of RORγ and Tbet, and (D) frequencies of indicated Th subsets. (E) Representative dot plots and frequencies of T_EM (CD62L^low) and T_CM (CD62L^high) among Th subsets. Unpaired t-test (+), Friedmann test followed by Dunn's test (*) and repeated measures one-way ANOVA followed by Tukey's test (#). *P < 0.05, **^++##P < 0.01, and ***P < 0.001, ****P < 0.0001.