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. 2019 May 9;216(6):1431–1449. doi: 10.1084/jem.20181078

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© 2019 Tanaka et al.

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Figure 8. — Regnase-1 ΔCTD mutation inhibits changes in its subcellular localization. (A) Immunoblotting of indicated proteins in subcellular organelles isolated from WT and Regnase-1^ΔCTD/ΔCTD MEFs after IL-17A stimulation for indicated times. (B and C) Analysis of polysome fractions isolated from WT and Regnase-1^ΔCTD/ΔCTD cell lysates stimulated with or without TNF-α and IL-17A for 4 h. (B) UV absorbance profile of sucrose-gradient fractions (10–50% sucrose) after ultracentrifugation of cell lysates. (C) Immunoblotting of Regnase-1 (Reg1) and RpL7a in sucrose-gradient fractions. Mw, molecular weight. NA, no addition.