Figure 2.

Nonlymphoid organs with high abundances of PD-1⁺ T conv cells were enriched in T reg cells. (A) Representative flow cytometric plots and summary data concerning T reg cell percentage at various ages (n = 3–9 mice/group). (B) Number of T reg cells in the liver of differently aged Aire^−/− mice (n = 4–9 mice/group). (C) Frequency of T reg cells in the spleen and various nonlymphoid organs of 8–10-d-old mice (n = 4–16 mice/group). (D) Correlation between frequencies of T reg cells (x axis) and PD-1⁺ T conv cells (y axis) in organs of 8–10-d-old Aire^−/− mice (median values of data shown in Figs. 1 G and 2 C; n = 4–16 mice/group). Data are pooled from two to four independent experiments and show mean ± SD. Statistical analyses in A–C as in Fig. 1. **, P ≤ 0.01; ***, P ≤ 0.001.