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. 2019 Apr 29;216(6):1411–1430. doi: 10.1084/jem.20182191

Figure 2.

Figure 2.

Microglial Fyn activation in human PD brains. (A) Significantly increased Fyn expression and activation were observed in AAV-SYN–injected nigral lysates, as assessed by immunoblots. Error bars represent mean ± SEM. Unpaired two-tailed t test (n = 4 mice per group). (B) IHC analysis revealed Fyn induction in Iba-1–positive microglia in AAV-SYN–injected nigral brain sections. Scale bars, 30 µm. (C) Significant increase in Fyn expression and activation in PD ventral midbrain lysates, when compared with age-matched control lysates. Representative immunoblot is shown. Error bars represent mean ± SEM. Unpaired two-tailed t test (n = 12). (D) IHC analysis of PD brain sections revealed strongly increased p-Y416 SFK expression within Iba-positive microglia when compared with age-matched non-PD brain sections. Error bars represent mean ± SEM. Unpaired two-tailed t test (n = 6). Scale bars, 15 µm. (E) IHC shows increased microglial Fyn expression in PD ventral midbrain sections. Scale bars, 15 µm. Error bars represent mean ± SEM. Unpaired two-tailed t test (n = 6). Asterisks indicate the level of statistical significance: *, P ≤ 0.05; ***, P ≤ 0.001.