Figure 1.

BCOR is required for optimal Th17 cell differentiation after Sp infection. WT and Bcor^fl/fl;Lck-cre⁺ (T cell BCOR-deficient) mice were infected with Sp-2W bacteria. After 7 d, 2W:I-A^b–specific CD4⁺ T cells were enriched from spleen and cervical lymph nodes using 2W:I-A^b tetramer and a magnetic based bead enrichment strategy. (A) B220⁻ CD11b⁻ CD11c⁻ CD4⁺ T cells from 2W:I-A^b tetramer–enriched samples with gates on CD44⁺ 2W:I-A^b tetramer⁺ cells. (B) Numbers of 2W:I-A^b–specific cells in individual WT and Bcor^fl/fl;Lck-cre⁺ mice with horizontal bars at the means. (C) Identification of 2W:I-A^b–specific (from gate in A) RORγt⁺ CXCR5⁻ Th17, RORγt^lo BCL6^lo Tfh, RORγt^lo BCL6^hi Tfh, RORγt⁻ BCL6^lo Tfh, RORγt⁻ BCL6^hi Tfh, TBET⁺ Th1, and FOXP3⁺ T reg cells. (D) Percentages of each subset among 2W:I-A^b–specific cells. Numbers in flow cytometry plots indicate percentages of gated populations. Data shown in all panels are representative of two independent experiments (n = 6–11 mice per group). Student’s t test; *, P < 0.05; ***, P < 0.001.