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. 2019 May 30;177(6):1619–1631.e21. doi: 10.1016/j.cell.2019.04.013

Figure 3.

Figure 3

Overall Structure of a 90A RNP-Pan2-Pan3 Complex

(A) Schematic representation of the domain organization of Pan2, Pan3, and Pab1. The different protomers and their respective domains visualized in the structure of the 90A RNP-Pan2-Pan3 complex are highlighted in different colors. Absent or untraceable regions are in white. Folded domains are shown as rectangles, and low-complexity sequences (such as the connecting segment CS of Pan2 or the Pab1 linker) are shown as bars. Numbers refer to the domain boundaries.

(B) Full cryoEM reconstruction of the Pan2-Pan3-90A RNP complex segmented and colored according to individual protomers or domains (in the case of Pab1 and Pan3 or in the case of Pan2 respectively, colors as in (A), with green showing the poly(A) RNP; see also Figure S2, Figure S3, Figure S4).

(C) The corresponding pseudo-atomic model of the Pan2-Pan3-90A RNP complex. The difference density (black mesh) shows the path of the poly(A) RNA. The pseudo-atomic model of the poly(A) RNA defines the overall binding path but cannot be interpreted in detail due to the limited resolution.