Figure S4.

Overview of the cryo-EM Data-Processing Scheme, Related to Figure 3

Particle sorting and classification tree used for 3D reconstruction of the Pan2-Pan3–90A RNP complex. The individual nominal global resolutions are quoted as good proxies for the translational and rotational accuracy of the reconstructions as well as for the level of detail observed in the individual maps. As outlined in the STAR Methods section and mentioned in Figure S3, three individual 3D reconstructions were mainly used for model fitting and structure interpretation. These are the reconstruction of the whole complex at a nominal overall resolution of 4.8 Å (Map 1), the reconstruction after signal subtraction of the Pan2-Pan3/1st Pab1 RRM1 and RRM2 complex at a nominal global resolution of 4.5 Å (Map 3) as well as the reconstruction of the Pan2-Pan3–90A RNP after signal subtraction of the UCH-like–RNase domain and RRM1 and 2 of the 1st Pab1 at a global resolution of 4.7 Å (Map 2). The masks used for signal subtraction are highlighted in red.