REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
α-gfp monoclonal antibody	Santa Cruz	sc-9996
goat α-mouse HRP polyclonal antibody	Bio-Rad	172-1011
Bacterial and Virus Strains
Rosetta (DE3) E.coli strain	Merck Millipore	70954
BL21 (DE3) E.coli strain	NEB	C2527l
Chemicals, Peptides, and Recombinant Proteins
S. cerevisiae Pab1 fl and derivatives	Schäfer et al., 2014; this paper	N/A
S. cerevisiae Pan2-Pan3 and derivatives	Schäfer et al., 2014; this paper	N/A
S. cerevisiae Ccr4-Caf1-Not1	Basquin et al., 2012	N/A
H. sapiens PAN2, PAN3 and PABPC1	This paper	N/A
Phusion High-Fidelity PCR Master Mix	NEB	M0531S
All used restriction enzymes	NEB	N/A
T7-polymerase	Produced in house	N/A
DNaseI (RNase-free)	Thermo Fisher Scientific	AM2222
T4 RNA ligase 1	NEB	M0204S
RNase A (Biochemistry Grade)	Ambion	AM2274
CIP	NEB	M0290S
T4 Polynucleotide Kinase	NEB	M0201S
Proteinase K	NEB	P8107S
ATP [γ-32P]	PerkinElmer	NEG002A100UC
pCp [5’-32P]	PerkinElmer	NEG019A250UC
acid phenol:chloroform	Ambion	AM9720
phenol:chloroform:isoamylalkohol (25:24:1)	Ambion	AM9730
chloroform:isoamylalkohol	SIGMA-ALDRICH	25668
NuPAGE 4-12% Bis-Tris protein gels	Thermo Fisher Scientific	NP0336BOX
8–16% stain-free protein SDS-PAGE gels	Bio-Rad	4568106
Sf-900 II SFM insect cell medium	Thermo Fisher Scientific	10902-104
Amersham ECL prime detection reagent	GE Healthcare	RPN2232
Deposited Data
EMDB	This paper	EMD: 4728
PDB	This paper	PDB: 6R5K
Experimental Models: Cell Lines
Trichoplusia ni Hi5 cells	Thermo Fisher Scientific	B85502
Spodoptera frugiperda Sf21 cells	Thermo Fisher Scientific	11497013
S. cerevisiae BY4743 strain	Euroscarf	http://www.euroscarf.de/index.php?name=News
Experimental Models: Organisms/Strains
isogenic to S. cerevisiae BY4741	This paper	WT
pan2-EGFP::kanMX4 in S. cerevisiae BY4741 background	This paper	Pan2-EGFP
(Δ1-340)-pan2-EGFP::kanMX4 in S. cerevisiae BY4741 background	This paper	Pan2ΔWD40-EGFP
(Δ1-340)-pan2-D1020A-EGFP::kanMX4 in S. cerevisiae BY4741 background	This paper	Pan2ΔWD40/cat-EGFP
Δpan2::klURA3 in S. cerevisiae BY4741 background	This paper	ΔPan2
Pan2::His3MX6 in S. cerevisiae BY4741 background	This paper	Pan2::His3
(Δ1-340)-pan2::His3MX6 in S. cerevisiae BY4741 background	This paper	Pan2ΔWD40
Oligonucleotides
homo-oligomeric poly(A) RNAs of defined length	ELLA Biotech	N/A
Recombinant DNA
ORFs cloned from genomic DNA of S.cerevisiae BY4743 strain and human IMAGE clones	Schäfer et al., 2014; http://www.imageconsortium.org/; this paper	N/A
pcDNA-3.1 (+)	Thermo Fisher Scientific	V79020
Software and Algorithms
MotionCor2	Zheng et al., 2017	https://msg.ucsf.edu/em/software/motioncor2.html
Gctf	Zhang, 2016	https://www.mrc-lmb.cam.ac.uk/kzhang/Gctf/
Gautomatch	N/A	https://www.mrc-lmb.cam.ac.uk/kzhang/Gautomatch/
RELION3.0	Zivanov et al., 2018	https://github.com/3dem/relion
sxviper	Penczek et al., 1994	http://sphire.mpg.de/
phenix.real_space_refine	Afonine et al., 2018b	https://www.phenix-online.org/
phenix.mtriage	Afonine et al., 2018a	https://www.phenix-online.org/
Coot	Emsley et al., 2010	https://www2.mrc-lmb.cam.ac.uk/Personal/pemsley/coot/
PyMOL2	N/A	https://pymol.org/2/
ChimeraX	Goddard et al., 2018	https://www.cgl.ucsf.edu/chimerax/
ConSurf 2016	Ashkenazy et al., 2016	http://consurf.tau.ac.il/2016/
ImageJ	N/A	https://fiji.sc/#
Tidyverse	N/A	https://www.tidyverse.org/
Other
Quantifoil holey carbon grids	Quantifoil	N/A
Superdex200 16/60 column	GE Healthcare	28-9893-35
Superose 6 3.2/300 column	GE Healthcare	29-0915-98
Superdex75 16/60 column	GE Healthcare	17-1068-01
Mono Q 5/50 GL column	GE Healthcare	17-5166-01
Strep-Tactin Sepharose	IBA lifescience	2-1201-002
Glutathion Sepharose 4 Fast Flow	GE Healthcare	17-5132-01
HIS-Select HF Nickel Affinity Gel	Sigma-Aldrich	H0537-25ML
Heparin Sepharose 6 Fast Flow	GE Healthcare	17-0998-01
GFPtrap agarose beads	Chromotek	Gta-20
Sepharose 4b	GE Healthcare	17-0120-01