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. 2019 May 28;10:1157. doi: 10.3389/fmicb.2019.01157

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Copyright © 2019 Nguyen, Saising, Tribelli, Nega, Diene, François, Schrenzel, Spröer, Bunk, Ebner, Hertlein, Kumari, Härtner, Wistuba, Voravuthikunchai, Mäder, Ohlsen and Götz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rom-resistance phenotype, genetic analysis and construction of deletion mutants and clones. (A) The Rom^R clone shows no inhibition halo in the agar diffusion method with 100 μg Rom loaded on the filter disks. (B) Genetic organization of the divers oriented farR and farE genes and location of the single point mutation (^∗) in farR leading to an amino acid exchange (Cys116/Arg) in the FarR regulator protein in the Rom^R clone. (C) Illustration of farE deletion construction in HG001 and the Rom^R clone. For homologous double-cross recombination, pBASE6-ΔfarE containing approximately 1 kb upstream and downstream DNA sequences were used; ΔfarE positive clones were controlled by PCR and sequencing. (D) pCX30::farR is the farR complementation plasmid in which the transcription of the gene is xylose-inducible.