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. 2019 Apr 5;10(7):1726–1733. doi: 10.7150/jca.26482

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Effects of P16-specific methylation on the survival of cancer cell lines treated with different doses of paclitaxel. (A) DHPLC chromatograms for the detection of fully methylated (P16M), partially methylated (pM), and unmethylated (P16U) P16 CpG islands (392 bp) in BGC823 cells stably transfected with a P16-specific DNA methyltransferase P16-Dnmt or the pTRIPZ control vector. P16-Dnmt expression was induced using doxycycline (dox) for different durations. (B, C) Quantitative RT-PCR and Western blot that detected the P16 expression levels in BGC823 cells. (D) MTT assay to detect the viability of P16-Dnmt stably transfected-BGC823 cells treated with various concentrations of paclitaxel. P16-Dnmt expression in these cells were pre-induced with doxycycline for 14 days. NC, negative control. ^*, P<0.05; ^**, P<0.01.