Figure 8.
Effects of peptide V3 on the PI, MVD, and apoptosis of human HCC xenografts. (A, B, C, D) Representive photographs of HE, Ki67, CD31, and Cleaved caspase-3 staining in SMMC-7721 and Huh-7 xenograft tumors; original magnification 400×. (E, F, G) The PI, MVD, and apoptotic index were calculated. The PI was calculated by the number of Ki67 positive cells among the total number of resting cells 25. Stained vessels with a clearly defined lumen or well-defined linear vessel shape were photographed and counted 22. Apoptotic index was calculated as cleaved caspase 3 positive cells/total cells 27. Values are presented as mean ± SEM (n = 6); **P < 0.01 compared with the control group; △△P < 0.01 compared with V1 group; ▲▲P < 0.01 compared with V2 group; ##P < 0.01 compared with V1+V2 group