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. 2019 May 28;10:567. doi: 10.3389/fphar.2019.00567

FIGURE 3.

FIGURE 3

TEA ameliorates ADR-elicited tubular cell injury. (A) Role of TEA in ADR-induced morphological changes. NRK-52E cells were pretreated with TEA (100 μg/mL) for 1 h and challenged with ADR for 24 h. Cell morphology was analyzed using phase-contrast microscopy (magnification, ×100). (B) Effects of TEA on cell viability. NRK-52E cells in 96-well plates were pretreated with TEA (100 μg/mL) for 1 h and challenged with ADR for 24 h. Cell viability was evaluated by the CCK-8 assay. Data are expressed as the percentages of living cells versus Ctrl (means ± SD, n = 5). ∗∗P < 0.01 versus Ctrl. ##P < 0.01 versus ADR in Ctrl. (C) Apoptosis staining of NRK-52E cells. NRK-52E in 48-well plates were pretreated with TEA (100 μg/mL) for 1 h and challenged with ADR for another 24 h. Apoptotic cells were evaluated by TUNEL and DAPI staining. Data on the right are expressed as the percentages of dead cells compared with the Ctrl (means ± SD, n = 5; ∗∗P < 0.01 versus Ctrl. ##P < 0.01 versus ADR in Ctrl). (D) The effects of TEA in flow cytometry assay. NRK-52E cells in 6-well plates were pretreated with TEA (100 μg/mL) for 1 h and challenged with ADR for another 24 h. The apoptotic cells were detected by flow cytometry to detect labeled Annexin V-FITC/PI. Flow cytometry analysis of apoptosis is shown on the right. ∗∗P < 0.01 versus Ctrl; ##P < 0.01 versus ADR in Ctrl.