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. 2019 May 28;10:567. doi: 10.3389/fphar.2019.00567

FIGURE 5.

FIGURE 5

TEA regulates ERK1/2 signaling. (A) Induction of p38 and ERK1/2 phosphorylation by ADR. NRK-52E cells in 12-well plates were incubated with ADR for 1, 3, 6, and 12 h Cellular lysates were analyzed by western blot for phosphorylated p38 and ERK1/2. Statistical analyses of phosphorylated ERK1/2 and p38 are shown at the bottom (means ± SD, n = 3; ∗∗P < 0.01 versus Ctrl). (B) Effects of TEA and NAC on ERK1/2 and p38 phosphorylation induced by ADR. NRK-52E cells in 12-well plates were pretreated with TEA (100 μg/mL) and NAC (5 mM) for 1 h and incubated with ADR for another 6 h. Lysates were analyzed by western blots targeting phosphorylated p38 and ERK1/2. Statistical analysis of phosphorylated ERK1/2 and p38 are shown at the bottom (means ± SD, n = 3; ∗∗P < 0.01 versus Ctrl; ## P < 0.01 versus ADR in Ctrl). (C) Effects of TEA on JNK phosphorylation induced by ADR. NRK-52E cells in 12-well plates were pretreated with TEA (100 μg/mL) and NAC (5 mM) for 1 h and challenged with ADR for another 6 h. Lysates were analyzed by western blot targeting phosphorylated JNK. Statistical analyses of phosphorylated JNKs are shown at the bottom.