Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 28;10:567. doi: 10.3389/fphar.2019.00567

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Li, He, Xia, Sun, Shi, Zhou, Zhu, Zhang, Liu, Zhu, Zhu, Zhou, Sun and Gao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

ADR induces oxidative stress-mediated NLRP3 inflammasome activation. (A) Induction of ERK1/2 phosphorylation and NLRP3 protein levels by ADR. NRK-52E cells in 12-well plates were incubated with ADR for 1, 3, 6, and 9 h, respectively. Statistical analyses of phosphorylated ERK1/2 and NLRP3 are shown at the bottom (means ± SD, n = 3; ^∗∗P < 0.01 versus Ctrl). (B) Effects of TEA and NAC on NLRP3 induced by ADR. NRK-52E cells in 12-well plates were pretreated with TEA or NAC for 1 h and incubated with ADR for 9 h. Statistical analyses of NLRP3 are shown at the bottom (means ± SD, n = 3; ^∗∗P < 0.01 versus Ctrl; ##P < 0.01 versus ADR in Ctrl). (C) Effects of TEA and U0126 on cell viability. NRK-52E cells in 96-well plates were pretreated with TEA (100 μg/mL) or U0126 (20 μM) for 1 h and challenged with ADR for 24 h. Cell viability was evaluated by CCK-8 assay. Data are expressed as percentages of living cells versus control (means ± SD, n = 5). ^∗∗P < 0.01 versus control; ## P < 0.01 versus ADR in Ctrl. (D) Effects of NLRP3 siRNA on ADR-induced cell injury. NRK-52E cells were transfected with either NLRP3 siRNA or control siRNA for 24 h. The transfected cells were incubated with ADR for 24 h. Cell viability was evaluated using a CCK-8 assay. Data are expressed as the percentages of living cells compared with the siRNA controls (means ± SD, n = 4; ^∗∗P < 0.01 versus siRNA control; ## P < 0.01 versus ADR in Ctrl).