Relationship between Marginal Patterning and Growth
(A and B) Localization of pPIN1::PIN1:GFP (green) in A. thaliana (A) and C. hirsuta (B) leaves at consecutive developmental stages.
(C–F) pPIN1::PIN1:GFP signal distribution near cell border (C and E) and growth anisotropy (D and F) in A. thaliana (C and D) and C. hirsuta (E and F) emerging protrusions.
(G–I) Heat-maps of area extension (G), growth anisotropy (H), and cell lobeyness (I) for cuc2-3 A. thaliana mutant.
(J and K) Heat-maps of area extension (J) and cell lobeyness (K) for 5 μm IAA treated A. thaliana leaves.
Yellow lines in (C) and (E) indicate cellular orientation of PIN1 polarization. White lines in (D), (F), and (H) indicate cell growth orientation when anisotropy > 20% (D and F) or 40% (H). Scale bars, 100 μm (A and B, G–K), 20 μm (C–F).
See also Figure S2, Figure S3, Figure S4, Figure S5 and Video S2.