Figure S4.

Leaf Growth Characteristics Converge in the Absence of Marginal Patterning, Related to Figures 2 and 3

(A) Silhouettes of A. thaliana wild-type (middle), cuc2-3 (left), and pin1 (right) mutant leaves.

(B-C) Heat-maps of cell proliferation (B), and cell size (C) for A. thaliana cuc2-3 leaf.

(D-G) Heat-maps of area extension (D), growth anisotropy (E), cell proliferation (F), and cell size (G) for the A. thaliana pin1 mutant leaf.

(H-J) Model of leaf growth in the absence of marginal patterning. (H) Specified growth is homogeneous and anisotropic during early development (initiation, 1-1.85 DAI), and follow indicated tissue-dependent directions thereafter (anisotropic in the midrib/petiole, isotropic in the blade). Specified growth rates depend on tissue type and differentiation. Differentiation begins when a compound diffusing from the leaf base (green curve, graph) falls below a threshold value (dotted line). Following differentiation, growth (orange curve) decreases. (I-J) Resultant shape and distribution of growth rates and anisotropy of the model (I), compared to growth anisotropy in cuc2-3 mutant (J). Note that growth rates decrease from the leaf tip to the base, mirroring the progression of differentiation. See also Video S3.

White lines in (E,J) indicate the orientation of cell growth where anisotropy is higher than 40%. Dotted lines in (C, G) indicate leaf outlines. Scale bars, 1 cm in (A), 100 μm in (B-G and I-J).