Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 10;218(6):1972–1993. doi: 10.1083/jcb.201807068

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Sharma and Subramaniam

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 1. — Rhes promotes filopodia-like cellular protrusions in striatal neuronal cells. (A) Striatal neuronal cells (STHdh^Q7/Q7) expressing GFP alone or GFP-RhoA or GFP-Rhes. Inset: Arrowheads show GFP puncta in the untransfected cells, and arrows show the filopodia-like process. DAPI indicates nuclei. (B) Bright field images (DIC) of striatal neuronal cells expressing GFP alone or GFP-RhoA or GFP-Rhes. Arrows point to filopodia-like protrusions. Inset, arrowheads indicate vesicle-like structures. (C) Bar graph shows data mean ± SEM; one-way ANOVA (***, P < 0.001). GFP alone (30.16 ± 1.68, n = 317), GFP-RhoA (35.44 ± 0.67, n = 301), and GFP-Rhes (67.56 ± 3.43, n = 300). (D) Confocal and DIC image of striatal neuronal cell expressing GFP-Rhes shows two different planes (substrate plane or above substrate). Arrow points to TNT-like cellular protrusion visible above the substrate plane from cell 1 to cell 2. (E) Bar graph shows data mean ± SEM; one-way ANOVA (***, P < 0.001). GFP alone (10.00 ± 1.52, n = 317), GFP-RhoA (13.05 ± 2.15, n = 301), and GFP-Rhes (30.98 ± 2.92, n = 300).