Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 10;218(6):1972–1993. doi: 10.1083/jcb.201807068

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Sharma and Subramaniam

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 8. — Rhes requires physical cell–cell contact to transport mHTT, which in acceptor cells associates with lysosome and other vesicles. (A) Experimental design for B and C. (B) FACS plot for the indicated co-cultured striatal neuronal cells. BFP cell population was gated, and 20,000 cells were recorded per sample. (C) Bar graphs show data mean ± SEM; Student’s t test (***, P < 0.001), n = 3, indicating percentage of BFP cells (acceptor cells) positive for Rhes + N171 89Q and Rhes + N171-89Q with Transwell (filter). (D) Experimental design for E. (E) Representative confocal and DIC images and their insets indicating donor cell (GPF-Rhes/BFP-mHTT) and acceptor cell (mCherry-tagged various organelle markers). Green channel shows GFP-Rhes, blue color represents BFP-mHTT, and red channel indicates the mCherry-tagged Lysosome (Lyso 20), Rab5a, Golgi-7, Peroxisome (Perox-2), and BIP individual organelle markers. Arrows indicate colocalization between BFP-mHTT and different organelle markers.