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. 2019 May 10;218(6):1972–1993. doi: 10.1083/jcb.201807068

Figure 9.

Figure 9.

Rhes transports Ataxin3 and poly 72Q protein but not mTOR or wtTau. (A and B) Representative confocal and DIC images of striatal neuronal cells expressing GFP-Rhes and mCherry-Ataxin 3 (Atxn3 84Q; A) or GFP-Rhes and mCherry-Poly 72Q (B). Arrows point to a TNT-like process. Arrowheads indicate GFP-Rhes colocalization with Atxn 84Q or Poly-72Q. (C) An experimental design for D and E. (D) Representative FACS plot for the indicated co-cultured cells. A total of 20,000 cells was recorded. (E–G) Bar graphs show data mean ± SEM; one-way ANOVA test (***, P < 0.001; **, P < 0.01), n = 3. Quantification of BFP cells (%) positive for both GFP and mCherry fluorescence (E), only GFP (F), and only mCherry (G). (H) FACS-sorted double-positive YFP-mTOR/mCherry alone or YFP-mTOR/mCherry-Rhes striatal neuronal cells (donor cells) were co-cultured with FACS-sorted BFP-expressing cells (acceptor cells). (I–K) Bar graphs show data mean ± SEM; Student’s t test (***, P < 0.001), n = 3. Quantification of BFP cells (%) positive for both YFP and mCherry fluorescence (I), mCherry only (J), or YFP only (K). (L) FACS-sorted double-positive (GFP alone/mCherry-Tau or GFP-Rhes/mCherry-Tau striatal neuronal cells [donor cells]) were co-cultured with FACS-sorted BFP-expressing cells (acceptor cells). (M–O) Bar graphs shows data mean ± SEM; Student’s t test (***, P < 0.001), n = 3. Quantification of BFP cells (%) positive for both GFP and mCherry fluorescence (M), GFP only (N), and mCherry only (O).