Table 1. Data collection and refinement statistics.
| Complex | |||
|---|---|---|---|
| Pdr6•RanGTP | Pdr6•Ubc9 | RanGTP•Pdr6•eIF5A | |
| PDB code | 6Q82 | 6Q83 | 6Q84 |
| Data collection | |||
| Space group | P6322 | R32 | P63 |
| Cell dimensions | |||
| a, b, c (Å) | 193.0, 193.0, 229.2 | 198.3, 198.3, 289.6 | 139.5, 139.5, 346.6 |
| α, β, γ (°) | 90.0, 90.0, 120.0 | 90.0, 90.0, 120.0 | 90.0, 90.0, 120.0 |
| Resolution (Å) | 59.89–2.99 (3.10–2.99) | 49.56–4.53 (4.69–4.53) | 49.17–3.70 (3.83–3.70) |
| Unique reflections | 51,160 (4,876) | 12,933 (1,242) | 40,583 (4,014) |
| Completeness (%) | 99.7 (97.4) | 99.41 (96.5) | 99.83 (99.7) |
| Rmerge (%) | 27 (776) | 17 (242) | 9 (292) |
| Rpim (%) | 1 (38) | 6 (83) | 3 (95) |
| I/σI | 44.70 (2.32) | 11.15 (0.95) | 17.74 (1.60) |
| CC1/2 | 100 (89.3) | 100 (58.5) | 100 (53.7) |
| Multiplicity | 399.8 (416.7) | 10.1 (9.4) | 10.8 (10.0) |
| Refinement | |||
| Resolution (Å) | 59.89–2.99 | 49.56–4.53 | 49.17–3.70 |
| No. reflections | 51,056 (4,876) | 12,933 (1,238) | 40,554 (4,014) |
| Rwork (%) | 21.8 (31.4) | 25.9 (40.1) | 20.7 (32.1) |
| Rfree (%) | 25.3 (35.7) | 27.1 (36.6) | 24.7 (33.4) |
| No. atoms | |||
| Protein | 9,649 | 9,380 | 21,141 |
| Ligand/ion | 33 | 66 | |
| B-factors | |||
| Protein | 111 | 311 | 197 |
| Ligand/ion | 91 | 179 | |
| RMSDs | |||
| Bond lengths (Å) | 0.003 | 0.005 | 0.002 |
| Bond angles (°) | 0.70 | 0.88 | 0.48 |
| MolProbity analysis | |||
| Ramachandran favored (%) | 94.24 | 93.64 | 94.00 |
| Ramachandran outliers (%) | 0.59 | 0.52 | 0.54 |
| Clash score | 11.05 | 10.79 | 6.18 |
| MolProbity score | 1.95 | 2.29 | 1.74 |
Statistics for the highest-resolution shell are shown in parentheses.