Figure 3.

Analysis of the energy production in Cre and CreTW chondrocytes. The Seahorse XF Cell Mito Stress assay was used to determine the bioenergetics profile in isolated chondrocytes from 1-d-old (n = 2 Cre, n = 3 CreTW) and 1-mo-old Cre and CreTW mice (n = 3). (A) The OCR over time was studied. (B) Basal respiration, ATP production-coupled respiration, and maximal and spare respiratory capacities were determined by adding the ATP synthase inhibitor oligomycin A (2 µM), the uncoupler of the RC FCCP (1 µM), and the inhibitors of complex III and I antimycin A (2.5 µM) and rotenone (0.5 µM), respectively (Coutelle et al., 2014). (C) The OCR and extracellular acidification rate were plotted to obtain the energetic profile of the chondrocytes. *P < 0.05, **P < 0.01.