Dithiolethiones inhibit NF-κB transcriptional activity. A, chemical structures of the dithiolethiones used in this study compared with ADT. B, H2S release from ACS-1 over 1200 seconds as measured by chemical potential electrode. C, MDA-MB-231 cells transiently transfected with an NF-κB luciferase reporter construct exposed to TNF-α (20 ng/mL) alone or in combination with ACS-1, ACS-2, NaSH, or VPA. Luciferase activity shown as mean RLU (± SD) was normalized to percent of TNF-α control activity.