Stacked mutations for simultaneous resistance to C17 and isoxaben. A, Root growth of 5-d-old wild type (WT) and progenies of BE3-CESA3S983F (S983F), ixr2-1, and double mutant ixr2-1 BE3-CESA3S983F (DM) in the absence (mock) or presence of 200 nm C17, 3 nm isoxaben (ISO), and the combination of 200 nm C17 and 3 nm isoxaben (C17 + ISO). Scale bars, 5 mm. B, Root length quantification of plants in A. Data represent mean ± sd (n > 4 plants). Statistically significant differences compared with plants in absence of C17 are indicated, *P < 0.01 (two-sided Student's t test). C, Morphology of 60-d-old wild type, S983F, and DM. Scale bar, 5 cm.