Figure 5.

Expression pattern of HvCEN under LDs. A and B, RNA in situ hybridization using the antisense probe. Red arrows indicate the HvCEN expression domains. C and D, Negative controls using the sense probe of HvCEN in the shoot apex of cv Bowman at Waddington stages 2.0 and 3.5. Scale bars = 100 µm. E, Expression of HvCEN in the pure inflorescence meristem of cv Bonus. Pure meristems, with six replicates per stage, were obtained by carefully removing leaf primordia and stem tissue beneath the inflorescence under the microscope. LDs are 16 h/8 h light/dark. W, Waddington stage. Error bars = sd.