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. 2019 Mar 20;180(2):896–909. doi: 10.1104/pp.18.01460

Figure 5.

Figure 5.

Auxin-regulated organ development in wild type and mab1-1. A and B, DR5rev:GFP expression in roots of wild type (Col; A) and mab1-1 (B). Arrowheads indicate expanded GFP signals. C and D, Immunolocalization of PIN1 and PIN2 in wild-type (Col; C) and mab1-1 (D) roots. E and F, DR5rev:GFP expression in heart-stage embryos of wild type (Col; E) and mab1-1 (F). G to J, PIN1 localization in the longitudinal middle (G and I) and surface (H and J) sections of wild-type (Col; G and H) and mab1-1 (I and J) embryos at the midheart stage. The arrow indicates the predicted direction of auxin transport by the polarity of PIN1 localization. K, Reverse transcription quantitative PCR analysis of cDNA from wild-type and mab1-1 roots in 5-d-old seedlings. Expression levels of PIN genes were unaffected by the mab1-1 mutation. Data shown are means of four independent experiments, with error bars representing the sd. L to O, PIN2-GFP localization in PIN2p:PIN2-GFP roots incubated with DMSO (L and N) or 500 mm 3-BP (M and O) for the indicated times. Scale bars = 20 µm.