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. 2019 Mar 20;180(2):896–909. doi: 10.1104/pp.18.01460

Table 1. Selected protein spots identified from the DIGE two-dimensional gels shown in Figure 3.

The mab1/wild type column shows the ratios of mab1-1 protein abundance to wild type (Col), with significant changes shown in the t test column; the experiment was performed with three replicates. Proteins whose spot numbers are indicated in Figure 3, C to E, were identified by matrix-assisted laser-desorption ionization time of flight tandem mass spectrometry. The predicted molecular mass and pI of the match are shown along with the molecular weight search score (P < 0.05, when the score is >37), number of peptides matched to tandem mass spectra, and percentage coverage of the matched sequence.

Spot No. Accession Description mab1/Wild Type t Test Score Matched Peptides Coverage (%) Mass pI
1 At5g50850 MAB1 7.28 0.0021 188 5 14.3 39436 5.67
2 At5g50850 MAB1 35.41 0.0003 290 5 14.3 39436 5.67
3 At5g50850 MAB1 −15.30 0.0017 138 3 11.3 39436 5.67
4 At5g50850 MAB1 −25.15 0.0015 298 6 14.3 39436 5.67
5 At1g59900 IAR4L −1.63 0.0011 183 3 8.5 43488 7.16
6 At1g24180 IAR4 −1.98 0.0034 175 4 12.2 43787 8.03
7 At1g03090 MCCA 2.33 0.0051 64 1 2.9 78776 6.31