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. 2019 Apr 10;180(2):859–873. doi: 10.1104/pp.19.00310

Figure 7.

Figure 7.

Knockdown of the coatomer subunits δCOP or εCOP results in mislocalization of GnTI. A, Left images show expression in the absence of any RNAi construct. “+δCOP” indicates coinfiltration with the N. benthamiana δCOP RNAi construct. The term “+εCOP” indicates coinfiltration with the N. benthamiana εCOP RNAi construct. mRFP-EMP12 (mRFP-tagged Arabidopsis EMP12 protein lacking the N-terminal luminal domain), NtGnTI-mRFP, ST-NtGnTI-mRFP, AtGnTI-mRFP, and AtGnTI-Q23A-mRFP were observed 48 h after infiltration using confocal microscopy. Scale bars = 25 μm. B, AtGnTI-Q23A-mRFP was treated with brefeldin A (100 μg/mL) for the indicated time. Scale bars = 25 μm. C, Immunoblot analysis of NtGnTI-mRFP, ST-NtGnTI-mRFP, AtGnTI-mRFP, and AtGnTI-Q23A-mRFP. Proteins were coexpressed with the indicated COP RNAi constructs, extracted 72 h after infiltration of N. benthamiana leaves and analyzed with anti-RFP antibodies. An arrowhead marks the intact fusion protein.