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. 2019 Mar 7;180(2):767–782. doi: 10.1104/pp.19.00059

Figure 7.

Figure 7.

RNAi-mediated knockdown of AADC1 in P. × canescens. Sterile P. × canescens calli were transformed via A. tumefaciens. Nontransgenic trees (WT, wild type), empty vector lines (EV-2-4), and RNAi lines (RNAi-1-4) were subjected to L. dispar feeding. Volatile 2-phenylethanol was collected from the headspace and analyzed using GC-MS/FID. Phenylethylamine and 2-phenylethyl-β-d-glucopyranoside were extracted with methanol from ground plant material and analyzed via LC-MS/MS. Biological replicates (nb) and technical replicates (nt) of EV lines and RNAi lines were used to test for statistical differences. Wild type, nb = 4; EV, nb = 3, nt = 5; RNAi, nb = 4, nt = 5. Asterisks indicate statistical significance as assessed by Student’s t test or Mann-Whitney Rank Sum tests. Phenylethylamine (P < 0.001, t = 7.940); 2-phenylethyl-β-d-glucopyranoside (P = 0.011, T = 547); 2-phenylethanol (P = 0.509, T = 404). Medians ± quartiles and outliers are shown. Each data point is represented by a circle. FW, fresh weight; n.s., not significant.