Figure 9.

Kinase activity and phosphorylation at Ser-209 and Thr-210 play roles in the BSK5 immune function. Wild-type (WT), bsk5, bsk5/BSK5-HA, bsk5/BSK5^K83E-HA (three lines), and bsk5/BSK5^S209A/T210A-HA (three lines) plants were treated with flg22 (A), elf18 (B), pep1 (C), or water and 24 h later were inoculated by infiltration with a suspension of Pst (1 × 10⁵ CFU mL⁻¹). Bacterial growth was measured at 0 and 4 dpi. Data are means ± se of three biological replicates each consisting of five plants. PTI was measured by subtracting bacterial growth at 4 dpi in PAMP/DAMP-treated plants from that in water-treated plants. Asterisks indicate significant differences compared with the PTI of wild-type plants based on Student’s t test (P < 0.05).