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. 2019 Mar 18;180(2):882–895. doi: 10.1104/pp.19.00142

Figure 7.

Figure 7.

Auxin distribution in root tips of the wild type (WT), mutants (m1 and m2), and overexpression lines (Ox1–Ox3). Seedlings were grown in nutrient solution containing 0.2 mM NO3− with or without 10 nm NAA application. A, Seminal root length. Plants were grown hydroponically for 14 d in nutrient solution containing 0.2 mM NO3− with/without 10 nm NAA application. B, pDR5::GFP (n = 30) expression in seminal root tips. Seedlings were grown for 7 d in nutrient solution containing 0.2 mM NO3− with or without 10 nm NAA application in agar medium. Bars = 1 mm. C to E, Plants were grown hydroponically for 14 d in nutrient solution containing 0.2 mM NO3−. C, IAA content in root tips of 0 to 0.5 cm and lateral root area of 2 to 8 cm adventitious root from the root tip (n = 7). D, [3H]IAA transport (n = 7). E, Relative expression levels of OsPINs. Relative mRNA levels for individual genes were normalized relative to OsActin (n = 3). Data in A and C to E are means ± se. *, P < 0.05 (Student’s t test) comparing the wild type and other lines. FW, Fresh weight.