Figure 1.

Glc regulates thermotolerance response in Arabidopsis. A–D, Percentage seedling survival, fresh weight, Chl content, and root-length measurement of 5-d–old MS-grown Col-0 seedlings treated without (0% Glc) or with increasing Glc (1% Glc, 3% Glc) and mannitol followed by HS. HS was applied as 1 h at 37°C, 2 h at 22°C, 2.5 h at 45°C, and 3–4 d at 22°C. Data shown are representative of one biological replicate having 25 seedlings. Experiment was repeated three times with similar results. E, Seedling survival phenotype of Col-0 seedlings without or with increasing Glc and mannitol. Images shown are representative of one biological replicate having at least 30 seedlings. Experiments were repeated three times with similar results. F, Percent seedling survival of 5-d–old MS-grown Col-0 and TOR overexpression (G166 and G548) seedlings treated without (0% Glc) or with Glc (3% Glc) followed by HS. Data shown are average of four biological replicates having at least 30 seedlings. G, Seedling survival phenotype of Col-0, G166, and G548 seedlings treated without or with Glc and subjected to HS. Images shown are representative of four biological replicates having at least 30 seedlings. Right shows schematic representation of HS treatment experimental setup. Experiments were repeated four times with similar results. Error bars = sd (Student’s t test, P < 0.05; *control versus treatment; **Glc versus mannitol/wild type versus overexpression).