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. 2019 Mar 19;180(2):1081–1100. doi: 10.1104/pp.18.01371

Figure 2.

Figure 2.

Mutation in E2Fa affects plant tolerance to HS. A, Seedling survival phenotype of Col-0 and e2fa-1 seedlings treated without or with Glc and subjected to HS. Images shown are representative of four biological replicates having at least 30 seedlings. Right shows schematic representation of HS treatment experimental setup. B, Percent fresh weight of 5-d–old MS grown Col-0 and e2fa-1 seedlings treated without (0% Glc) or with Glc (3% Glc) followed by HS. Data shown are average of four technical replicates having at least 20 seedlings. Experiments were repeated two times with similar results. C, Percent Chl content in Arabidopsis Col-0 and e2fa-1 seedlings in presence or absence of Glc and HS. Data shown are average of three biological replicates having at least 30 seedlings. Experiments were repeated three times with similar results. D, Percent lateral root number of Col-0 and e2fa-1 seedlings in the presence of Glc and HS. Data shown are average of three biological replicates having at least 20 seedlings. E, RT-qPCR expression of HS-related genes in Arabidopsis Col-0 and E2Fa-DPa overexpressing (E2Fa-DPa) plants. Four-week–old Col-0 plants were transiently coinfiltrated with 35SCaMV::E2Fa and 35SCaMV::DPa constructs followed by HS. HS was applied as 3 h at 37°C and harvested immediately. DPa transcription factor is known to activate E2Fa activity. Experiments were repeated two times with similar results. Ctrl, control. Error bars = sd (Student’s t test, P < 0.05; *control versus treatment; **wild type versus mutant/overexpression).