Figure 9.

Glc mediates thermomemory response. A, RT-qPCR expression of thermomemory-related genes in Arabidopsis Col-0 seedlings treated without (0% Glc) and with Glc (3% Glc) followed by HS. HS was applied at 1 h at 37°C, 90 min at 22°C, and 45 min at 45°C. After HS, seedlings were recovered for various time points (3 h, 24 h, and 48 h) at 22°C. Data shown are representative of one biological replicate. Experiments were repeated two times with similar results. B, ChIP-qPCR of thermomemory-related genes in Col-0 seedlings treated without or with Glc followed by HS. HS was applied as 1 h at 37°C, 90 min at 22°C, and 45 min at 45°C. After HS, seedlings were recovered for 24 h at 22°C. Amount of immunoprecipitated promoter DNA was calculated by comparing samples treated without or with anti-Histone H3K4me3 antibody. Data shown are representative of one biological replicate. Experiments were repeated two times with similar results. CT values without and with anti-Histone H3K4me3 antibody were normalized by input control. Rec, recovery after HS; Ctrl, control. Error bars = sd (Student’s t test, P < 0.05; *control versus treatment).