Figure 3.

Organ-specific expression analysis of auxin-related and growth-related genes. A, Seedlings cultivated for 7 d at 20°C were transferred to 20°C or 28°C at zeitgeber time 16 (ZT16) for 8 h prior to harvesting cotyledons and hypocotyls for expression analysis. Seedlings were either dissected after or before the temperature shift, including removal of the root. B, Reverse transcription quantitative PCR (RT-qPCR) expression analysis of the auxin biosynthesis gene YUC8 was assessed for cotyledons and hypocotyls. C, Genes relevant for hypocotyl elongation (SAUR19 and SAUR20) and an auxin response gene (IAA19) were assessed in hypocotyl samples. Seedlings were grown in long-day conditions (16/8 h) under 30 µmol m⁻² s⁻¹ white fluorescent light. RT-qPCR analyses were performed on three independent biological replicates. Bar plots show mean values, and error bars denote se. Different letters denote statistical differences at P < 0.05 as assessed by one-way ANOVA and Tukey’s HSD posthoc test.