Figure 2.

Histological and cellular localization of CsSUT1. A–N, Immunolocal detection of CsSUT1 in stage 10 male flowers using a CsSUT1-antiserum. A, C, E, G, I, K, L, N, Immunolabel detected in the tapetum and microspores in anthers, and phloem cells in sepals, petals, filaments, and the pedicel. B, D, F, H, J, M, Control sections incubated with preimmune serum were devoid of label. (G), (H), (K), and (N) are transverse sections, and the others are longitudinal sections. (L), (M), and (N), are the close-ups of (I), (J), and (K), respectively. O, Plasma membrane localization of CsSUT1-GFP fusion protein in protoplasts derived from cucumber cotyledons. GFP served as a control. Abbreviations: T, tapetum; M, microspore; Ph, phloem; Xy, xylem; SE, sieve element; PM, plasma membrane; Auto, chlorophyll autofluorescence. Scale bars = 50 μm in (A–N) and 5 μm in (O).