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. 2017 Jun 21;10(4):305–310. doi: 10.1080/21541248.2017.1324940

Figure 2.

Figure 2.

EphA2 receptor expressed on RasV12 cells is required to drive RasV12 cell repulsion and contractility following interaction with normal cells. Confocal images of coculture assays. Upper panels: Normal MDCK cells prelabeled with cell tracker dye (CMDRA) are added to confluent monolayers of GFP-RasV12 cells constitutively expressing EphA2 shRNA. Middle panels: GFP-RasV12 cells prelabeled with cell tracker dye (CMDRA) are added to confluent monolayers of GFP-RasV12 cells constitutively expressing EphA2 shRNA. Lower panels: GFP-RasV12 cells constitutively expressing EphA2 shRNA prelabeled with cell tracker dye (CMDRA) are added to confluent monolayers of normal MDCK cells. Cells were fixed 24 h after addition of prelabeled cells and stained with phalloidin (gray), anti-EphA2 antibody (magenta) and Hoechst (blue). XY images represent maximum projections of z-stacks. Scale bar, 20 μm.